![]() ![]() In this study, we have systematically evaluated and explored the use of a multiplex bead-based flow cytometric assay which is compatible with most standard flow cytometers and facilitates a robust semi-quantitative detection of 37 different potential EV surface markers in one sample simultaneously. Unfortunately, such methods generally require dedicated instrumentation, are time-consuming and demand extensive operator expertise for sample preparation, acquisition, and data analysis. Within recent years, several new methods and assays to study EV heterogeneity in terms of surface markers have been described most of them are being based on flow cytometry. ![]() For instance, the surface marker profile of EVs is likely dependent on the cell source, the cell’s activation status, and multiple other parameters. Nowadays, it is however commonly accepted in the field that there is a much higher degree of heterogeneity within these two subgroups than previously thought. EVs can be conceptually classified based on their size and biogenesis as exosomes and microvesicles. 10Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United KingdomĮxtracellular vesicles (EVs) can be harvested from cell culture supernatants and from all body fluids.9Department of Pediatrics III, University Children’s Hospital of Essen, University Duisburg-Essen, Essen, Germany.8Department of Otorhinolaryngology & Head/Neck Surgery, University Hospital Düsseldorf, Heinrich Heine University of Düsseldorf, Düsseldorf, Germany.7Department of Biology, College of Science, Salahaddin University-Erbil, Erbil, Iraq.6Department of Clinical Neuroscience, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden.5Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.4Division of Pathology F56, Department of Laboratory Medicine, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden.3Molecular Immunogenetics and Vaccine Research Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, United States.2Evox Therapeutics Limited, Oxford, United Kingdom.1Clinical Research Center, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden.Samir EL Andaloussi 1,2,10† and André Görgens 1,2,5* † ![]()
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